Scientist: Prof Amrita Ahluwalia, Clinical Pharmacology, William Harvey Research Institut, Barts & The London Medical School. Aim: To investigate the effects of the ‘Suron light device’ on human endothelial cells. Endothelial cells are the cells that form a single cell layer lining the luminal side of all blood vessels. These cells play an essential role in maintaining the healthy state of the blood vessel by ensuring adequate provision to vital organs of nutrients and oxygen and by releasing substances that maintain the patency of blood vessels and prevent blockages (as those that precipitate heart attacks). In addition, these are the cells involved in the formation of new blood vessels (angiogenesis). Method: We plated human endothelial cells into 6-well culture plates and counted the cells to monitor proliferation. Endothelial cells will proliferate over time  such that seeding of a small number of cells onto a culture plate over approximately 4 days will proliferate to cover the culture plate area. The rate of growth can be monitored by counting the number of cells at set times after ‘seeding’ which is how we estimated growth rates in this study. We conducted two types of experiment. In the first series of experiments the cells received continuous ‘light’ for the first 48 hours of growth. This experiment was halted due to the fact that all cell growth was prevented by the light devices. In the second series of experiments the cells were allowed to reach an active growth phase (72 hours, see figure 1 for normal growth pattern) and were then subjected to 12 hours of ‘light’. Plates were left to grow in a temperature (37oC), humidity and 5% CO2 in atmospheric O2 environment in an incubator as control. In a separate incubator cells were subjected to the ‘Suron light device’ which was placed on top of the plate. At the end of this time period cells were collected and counted. Cell viability was assessed by trypan blue staining. Results: Normal growth of cultured endothelial cells is shown in Figure 1. Figure 1: Typical cell growth cycle of human endothelial cells Continuous exposure to the light device light from time zero inhibited cell growth completely at 48 hours compared with controls, table 1. In light of this the experiment was modified.
 
No wellcell
wellcell
0 hours
48750
48750
24 hours
24707 ± 2143 N=2
21200 ± 1346 N=2
48 hours
62409 ± 847 N=2
15593 ± 1934 N=2
Table 1: Cell growth following continuous exposure to the wellcell. Values are mean ± SEM
A further experiment was designed to allow time for the cells to reach an active growth period. At 72 hours growth the cells received 12 hours of exposure to the wellcell. The growth of the cells was markedly reduced when compared with control (70000 ± 17320 N=4 vs. 285000 ± 53770 N=4 respectively, p=0.0089), Figure 2. Further investigation also showed the Wellcell to decrease cell viability, i.e. to increase cell death when compared with control (73.00% ± 2.00% N=4 vs. 93.50% ± 3.12% N=4 respectively, p = 0.0015), Figure 3.
Figure 2: Number of cells with and without Wellcell light exposure.
Figure 3: Cell viability of cells with and without Wellcell light exposure.
Conclusions: These   experiments   demonstrate   a   profound   inhibitory   effect   of   the   wellcell   on   endothelial   cell   growth.   These   findings   would   suggest   that   the   wellcell would   be   effective   in   inhibiting   new   vessel   growth.      The   potential   therapeutic   utility   of   such   an   effect   would   likely   fall   in   the   area   of   cancer,   where   new vessel   growth   perpetuates   tumour   growth   and   survival.      Inhibition   of   new   vessel   growth   would   theoretically   deprive   the   tumour   of   blood   flow   and   thereby inhibit further growth.
Recommendations: The   wellcell   should   be   tested   both   on   tumour   growth   models   but   also   upon   models   assessing   new   vessel   growth   associated   with   tumour   growth. However   the   profound   effect   on   endothelial   cell   growth   needs   to   be   viewed   with   caution.     The   profound   inhibition   of   cell   growth   suggests   a   highly   potent effect that potentially could lead to detrimental effects.
 Study: To investigate the effects of Suron Light device on Human endothelial cell growth
Cancer Research
Wellcell   has   by   testing   their   product   on   Thoroughbred   racehorses   the   elite   racing   machine. A   few   of   our   clients   owned   race   horses   and   because   they   felt so   well   using   their   Wellcell,   they   thought   if   they   treated   their   horses   with   a   Wellcell   that   they   could   get   a   racing   advantage.   When   we   counted   up   all   the horses   we   were   treating   and   how   many   race   starts   they   had,   we   discovered   that   our   winning   racing   statistics   were   way   above   the   average. This   proved   a game   changer   for   Wellcell   as   the   owners   horses   performed   so   well   that   their   trainers   put   Wellcells   on   all   their   other   stable   horses.   We   had   several impressive   group   1   winners   which   is   the   pinnacle   for   every   owner/trainer   and   decided   we   should   do   a   trial   in   a   big   Sydney   racing   stable.   Check   out   the video  above. We   were   so   impressed   with   the   trial   results   on   horses   we   had   a   chance   to   trial   Wellcell   on   a   young   teenage   swimmer.   She   was   your   normal   average   club swimmer   and   the   results   we   achieved   were   quite   astonishing   as   she   started   putting   lengths   ahead   of   her   other   club   mates.   Parents   of   the   other   club swimmers   noticed   her   performance   and   asked   Angela’s   mother   what   she   was   doing   to   make   her   go   so   well.   Her   mother   obviously   didn’t   tell   them because it was her big secret. She   went   from   your   ordinary   club   swimmer   to   New   Zealand   club   champion   for   her   age   group.   She   won   every   swimming   event   at   her   high   school   and even won every athletic event she entered at the school championships. We   approached   the   New   Zealand   sports   institute   to   do   a   trial   but   unfortunately   they   wanted   to   be   paid   a   prohibitive   amount   of   money   for   their   services and at the time we were still in the hardware development stage.
How Do You Quantify any Claim That You Get Energised From A Product?
Scientist: Prof Amrita Ahluwalia, Clinical Pharmacology, William Harvey Research Institut, Barts & The London Medical School. Aim: To investigate the effects of the ‘Suron light device’ on human endothelial cells. Endothelial cells are the cells that form a single cell layer lining the luminal side of all blood vessels. These cells play an essential role in maintaining the healthy state of the blood vessel by ensuring adequate provision to vital organs of nutrients and oxygen and by releasing substances that maintain the patency of blood vessels and prevent blockages (as those that precipitate heart attacks). In addition, these are the cells involved in the formation of new blood vessels (angiogenesis). Method: We plated human endothelial cells into  6-well culture plates and counted the cells to  monitor proliferation. Endothelial cells will proliferate  over time such that seeding of a small number of cells onto a culture plate over approximately 4 days will proliferate to cover the culture plate area. The rate of growth can be monitored by counting the number of cells at set times after ‘seeding’ which is how we estimated growth rates in this study. We conducted two types of experiment. In the first series of experiments the cells received continuous ‘light’ for the first 48 hours of growth. This experiment was halted due to the fact that all cell growth was prevented by the light devices. In the second series of experiments the cells were allowed to reach an active growth phase (72 hours, see figure 1 for normal growth pattern) and were then subjected to 12 hours of ‘light’. Plates were left to grow in a temperature (37oC), humidity and 5% CO2 in atmospheric O2 environment in an incubator as control. In a separate incubator cells were subjected to the ‘Suron light device’ which was placed on top of the plate. At the end of this time period cells were collected and counted. Cell viability was assessed by trypan blue staining. Results: Normal   growth   of   cultured   endothelial   cells   is   shown in Figure 1. Figure 1: Typical cell growth cycle of human endothelial cells Continuous exposure to the light device light from time zero inhibited cell growth completely at 48 hours compared with controls, table 1. In light of this the experiment was modified.
 Study: To investigate the effects of Suron Light device on Human endothelial cell growth
Cancer Research
 
No wellcell
wellcell
0 hours
48750
48750
24 hours
24707 ± 2143 N=2
21200 ± 1346 N=2
48 hours
62409 ± 847 N=2
15593 ± 1934 N=2
Table 1: Cell growth following continuous exposure to the wellcell. Values are mean ± SEM
A further experiment was designed to allow time for the cells to reach an active growth period. At 72 hours growth the cells received 12 hours of exposure to the wellcell. The growth of the cells was markedly reduced when compared with control (70000 ± 17320 N=4 vs. 285000 ± 53770 N=4 respectively, p=0.0089), Figure 2. Further investigation also showed the Wellcell to decrease cell viability, i.e. to increase cell death when compared with control (73.00% ± 2.00% N=4 vs. 93.50% ± 3.12% N=4 respectively, p = 0.0015), Figure 3.
Figure 2: Number of cells with and without Wellcell light exposure.
Figure 3: Cell viability of cells with and without Wellcell light exposure.
Conclusions: These      experiments      demonstrate      a      profound inhibitory   effect   of   the   wellcell   on   endothelial   cell growth.    These    findings    would    suggest    that    the wellcell   would   be   effective   in   inhibiting   new   vessel growth.      The   potential   therapeutic   utility   of   such   an effect   would   likely   fall   in   the   area   of   cancer,   where new   vessel   growth   perpetuates   tumour   growth   and survival.        Inhibition    of    new    vessel    growth    would theoretically   deprive   the   tumour   of   blood   flow   and thereby inhibit further growth.
Recommendations: The    wellcell    should    be    tested    both    on    tumour growth   models   but   also   upon   models   assessing new   vessel   growth   associated   with   tumour   growth. However    the    profound    effect    on    endothelial    cell growth    needs    to    be    viewed    with    caution.        The profound   inhibition   of   cell   growth   suggests   a   highly potent     effect     that     potentially     could     lead     to detrimental effects.
How Do You Quantify any Claim That You Get Energised From A Product?
Wellcell      has      by      testing      their      product      on Thoroughbred      racehorses      the      elite      racing machine.   A   few   of   our   clients   owned   race   horses and   because   they   felt   so   well   using   their   Wellcell, they   thought   if   they   treated   their   horses   with   a Wellcell   that   they   could   get   a   racing   advantage. When    we    counted    up    all    the    horses    we    were treating   and   how   many   race   starts   they   had,   we discovered   that   our   winning   racing   statistics   were way    above    the    average.    This    proved    a    game changer     for     Wellcell     as     the     owners     horses performed   so   well   that   their   trainers   put   Wellcells on   all   their   other   stable   horses.   We   had   several impressive   group   1   winners   which   is   the   pinnacle for   every   owner/trainer   and   decided   we   should   do a   trial   in   a   big   Sydney   racing   stable.   Check   out   the video  above. We   were   so   impressed   with   the   trial   results   on horses    we    had    a    chance    to    trial    Wellcell    on    a young   teenage   swimmer.   She   was   your   normal average     club     swimmer     and     the     results     we achieved   were   quite   astonishing   as   she   started putting    lengths    ahead    of    her    other    club    mates. Parents   of   the   other   club   swimmers   noticed   her performance   and   asked Angela’s   mother   what   she was   doing   to   make   her   go   so   well.   Her   mother obviously   didn’t   tell   them   because   it   was   her   big secret. She   went   from   your   ordinary   club   swimmer   to   New Zealand   club   champion   for   her   age   group.   She won   every   swimming   event   at   her   high   school   and even   won   every   athletic   event   she   entered   at   the school championships. We   approached   the   New   Zealand   sports   institute to   do   a   trial   but   unfortunately   they   wanted   to   be paid    a    prohibitive    amount    of    money    for    their services    and    at    the    time    we    were    still    in    the hardware development stage.